Stimulation of phenolic compounds accumulation and antioxidant activity in in vitro culture of Salvia tebesana Bunge in response to nano-TiO2 and methyl jasmonate elicitors

In the present study, we first attempted to achieve an efficient procedure for optimizing callogenesis from apical meristem and leaf explants of Salvia tebesana on MS media containing different concentrations BAP alone in combination with 2,4-D. Then, inducing effect nano-TiO2 (10, 60, 120 mg L−1) methyl jasmonate (50, 100, 200 µM), as abiotic elicitors were studied enhancement phenolic compounds, rosmarinic acid, some individual flavonoids well antioxidant capacity callus. According results, highest rate (100 93.33, respectively) DW (0.55 ± 0.03 0.36 0.02 g, per responsive explant achieved "BAP1 + 2,4-D1" L−1 medium supplemented "BAP0.5 L−1. The elicitation 10 60 (respectively leaf), 50 µM MeJa could significantly promote production predominant derivatives S. calli, where content total phenolics, O-diphenols, flavonoid, flavone flavonol, proanthocyanidin was recorded. As results declared that increasing acid more correlated treatment than elicitation. Moreover, apigenin (0.33 μg g−1 DW) detected after MeJa-elicitation (50 while maximum level quercetin (2.62 0.09 rutin (13.79 0.87 obtained exposure nano-TiO2, both leaf-derived calli. While a significant positive correlation recorded between assays (DPPH FRAP) treated calli; very strong occurred meristem-derived calli DPPH FRAP values (r2 = − 0.921 r2 0.913, P < 0.01 respectively). Our showed vitro culture would be good technique successfully produce enhance pharmacologically valuable metabolites tebesana. Nano-TiO2 concentration-dependent manner increased specific flavonoids, properties callus Bunge.